Assessing garden pea germplasm for powdery mildew resistance through disease phenotyping and genotyping using molecular markers

Erysiphe pisi is one among the other causal agents of powdery mildew infection in garden pea. So far, resistance genes comprising two recessive (er1 and er2) dominant (Er3) have been reported to confer A set 46 pea genotypes were screened against E. isolate- Ep01 greenhouse conditions identify resistant genotypes. Disease reaction genotyping carried out test for resistance/ susceptibility through gene-specific sequence characterized amplified region (SCAR) markers. The presence alleles control genotypes- JI2302 er1 gene, JI2480 er2 gene P660–4 Er3 was confirmed their respective genotype- Arkel served as a negative control. Screening germplasm revealed 3 highly resistant, 6 while 10 moderately 13 susceptible, 9 susceptible 5 susceptible. molecular marker Sc-OPE-161600 found be segregating most except control- Arkel. marker- ScX17_1400 homozygous condition associated with resistance, SCW4637, heterozygous majority P660–4.